Influence of murine cytomegalovirus infection on the differentiation and the differentiation genes expression in neural stem cells

AIM: The influence of MCMV infection on differentiation and differentiation gene expression in neural stem cells(NSCs) in vitro were investigated for studying the mechanisms of brain abnormalities caused by congenital cytomegalovirus infection.METHODS: NSCs were separated from fetal BALB/C mouse, and cultured and identified in vitro. The differentiation potency of NSCs was observed by immunofluorescence. The NSCs infected by MCMV at dosage of MOI(multiplicity of infection) equaled to 5, 1 and 0.1,respectively, were cultured in differentiation medium. The morphological changes of infected cells were observed under inverted microscope. The ratios of NSCs and its differentiated cells were detected by flow cytometry. The expressions of nestin, GFAP and NSE, markers of NSCs and its differentiated cells, were studied by immunofluorescence(MOI=1). The expression of early antigen(EA) of MCMV was detected to observe the infection process. Real-time RT-PCR method was employed to measure the expression levels of the key genes Neurog2, Myc and Ccnd1 in Wnt signal pathway of NSCs at early stage of differentiation culture.RESULTS: NSCs isolated from embryonic mouse brains proliferated to form neurospheres, strongly expressed nestin and differentiated into NF-200 positive neurons or GFAP positive astrocytes. The infected NSCs did not adhere to the wall and appeared differentiation growths, but showed swollen gradually after differentiation culture. The nestin expression in the infected cells downregulated slowly and was higher than that in control groups(P<0.05). The GFAP and NSE expressions of the infected cells were lower than those in control groups(P<0.05). The early antigen(EA) of MCMV was always detected in the cells in infected groups. The ratios of nestin positive cells in infected groups were higher than those in control groups, but the ratios of GFAP and NSE positive cells of former were lower than that of the latter from 3rd to 9th d after differentiation culture(P<0.05). The levels of Neurog2 mRNA and Myc mRNA in infected groups were markedly lower than those in normal control groups on 1st d and from 1st to 4th d after differentiation culture, respectively(P<0.05). The levels of Ccnd1 mRNA of infected groups were obviously lower than those in normal control groups from 12th h to 1st d(P<0.05). These changes in infected groups became more obvious as MCMV MOI increased.CONCLUSION: MCMV significantly inhibits differentiation of NSCs to neurons and astrocytes, and leads to the decrease in differentiated cells. MCMV inhibits or interferes with the gene expression of Neurog2, Myc and Ccnd1 in Wnt signal pathway of NSCs. The effect that MCMV inhibits the expressions of differentiation and the differentiation genes in NSCs shows dose-dependent with MCMV MOI. The inhibitory effect of MCMV on the differentiation of NSCs might be induced by interfering with the expression of differentiation gene in NSCs, which is possibly the one of primary causes of brain development disorders induced by congenital CMV infection.     

菠菜品种比较试验

对引进的法莲草、胜先锋、黄菠、全能、超越、墨丽、无敌、急先峰、春夏、赤虎耳等10个菠菜品种进行植物学性状、产量、抗病性的分析比较试验。试验结果表明，超越和黄菠两个品种具备产量高、品质优、抗病性强等优点，可作为福州市秋、冬菠菜的主栽品种。     

农林环境机器视觉导航路径生成算法及应

提出两种自然环境中的路径导航线生成算法:对于矮小作物规则分布的农田场景,在标准Hough变换的基础上,预先检测共线点峰值的限定偏角阈值,以迅速检测关键信息;对于林地环境及类林地环境的高大作物农田场景,寻找树干与地面的交点,形成机器人行走的左右边界,再求两边交点的中值产生一列点簇.对该列点簇进行Hough变换检测直线作为导航线,或应用最小二乘法拟合左右边界,求其中线作为路径导航线.Matlab对比仿真表明,几种算法对各自适用的场景具有可靠稳定的路径辨识能力,可对图像进行有效的批量处理.     

中国农产品地理标志管理制度发展方略思考

地理标志(georaphical indications)是指标示来源于某地区,该商品的特定质量、信誉或者其他特征,主要由该地区的自然因素或者人文因素所决定的标志.中国是个传统农业文明古国,悠久的农业文明孕育了丰富的农产品地理标志资源,虽然中国在地理标志制度建设上做了一些有益的尝试,但农产品地理标志的保护和管理水平与国外成熟方式比较起来,差距还很大.……     

2006年贵州省赫章县秋季小麦条锈病调查

为了解中国新发现的小麦条锈病菌越夏区贵州省赫章县小麦条锈病秋季发生情况,2006年秋季笔者主要对该县古基乡、野马川镇、六曲河镇进行了调查,共调查119块地,自生麦病田率为55%,秋播小麦病田率为20.20%。古基乡发病较多,自生麦苗上有多个发病中心,有的秋播小麦田块亦呈现病叶较集中现象。在海拔1660-1910m范围内,都有小麦条锈病发生,说明当地越夏菌源能够为本地区秋苗发病提供充足菌量。对进一步研究内容进行了讨论并提出了赫章县小麦条锈病治理措施。     

国内外辐照食品现状及我国的发展对策

本文就目前国内外辐照食品的现状进行了概述,并对我国辐照食品存在的问题及发展对策进行了简单的分析。     

在线清筛机构在粉碎机中的运用

<正>1现有粉碎机的运行状态及分析物料经锤片打击破碎后加速作圆周运动,离心力的作用使物料脱离锤片的打击,并很快在筛片侧形成物料环,粗细物料的质量不同造成物料环的分层,粗料粒层紧靠筛片侧,细料粒层覆在粗料粒层上。如果无法破坏物料的环流层,不但影响大颗粒物料重返粉碎区,而且还影响细物料的过筛,     

牙鲆血清免疫球蛋白的分离纯化及部分特性分析

运用Sephacryl S-200凝胶层析和HiTrap rProtein ASepharose亲和层析2种方法对牙鲆(Paralichthys olivaceus)血清免疫球蛋白进行分离纯化,结果表明,牙鲆免疫球蛋白分布于33%~50%的硫酸铵饱和溶液中,其中45%的分离效果最好。凝胶层析和亲和层析样品均出现2个蛋白峰,用还原SDS-PAGE检测确定牙鲆免疫球蛋白存在于第2个蛋白峰中。牙鲆免疫球蛋白重链分子量约为75.4 kD,轻链分子量约为29.9 kD和28.2 kD,推测牙鲆血清免疫球蛋白的分子量为836 kD。制备了兔抗牙鲆免疫球蛋白多克隆抗体,免疫双扩散法检测多克隆抗体效价为1∶32,免疫斑点法检测多克隆抗体效价至少为1∶1 600。运用免疫印迹法(Western-bloting)检测了兔抗牙鲆免疫球蛋白多克隆抗体的特异性,实验证明该抗体与牙鲆全血清中免疫球蛋白重链、轻链反应均成阳性。     

干法保存30年的鲤鱼脑垂体催情异育银鲫产卵成功

首次报道用干法保存了30年的鲤鱼脑垂体催情异育银鲫产卵成功。当该垂体的剂量分别为10、20、30mg/kg亲鱼体重时,各获得50%、70%、90%的催产率,以及60.08±0.49%、70.42±0.32%、75.27±0.28%的受精率和79.93±0.29%、80.46±0.55%、81.10±0.26%的孵化率,平均每尾约250g体重的亲鱼产异育银鲫鱼苗0.976万尾。这些鱼苗和正常对照组的鱼苗一样,已经培育成体格健壮的优质鱼种。     

Serum concentration of S100-B protein in patients during CABG with or without cardiopulmonary bypass

AIM: To investigate the mutative law of the serum S100-B protein in patients undergone coronary artery bypass grafting with or without cardiopulmonary bypass (CPB-CABG or non-CPB CABG), and to compare the degree of cerebral injury in patients during CABG between the two different surgical manipulations.METHODS: 30 patients were divided into two groups: CPB-CABG group (group A) and non-CPB CABG group (group B). Every group included 15 patients. Blood from jugular vein was phlebotomized in every patients at the following 8 time points: before operation (OP), after anaesthesia, end of grafting, end of OP, 2 h after OP, 6 h after OP, 12 h after OP and 24 h after OP. The serum concentrations of S100-B protein in the samples were measured.RESULTS: The level of serum S100-B protein increased markedly at the beginning of CABG, and the peak value of group A (2.32 μg/L±0.26 μg/L) was treble higher than that in the patients in group B (0.71 μg/L±0.14 μg/L). The levels of S100-B protein tend to normal level in both groups 24 h after OP. The level of S100-B protein had notable relation with the time of CABG in group A and the manipulative time on the heart in group B.CONCLUSION: The degree of cerebral damage can be valued by investigating the mutative law of the serum S100-B protein in patients undergone CPB-CABG and non-CPB CABG. The non-CPB CABG reduces the degree of cerebral damage and avoids the functional disorder of central nerve.